A clean machine -Reply

Florence Harrod fharrod at pharmingen.com
Wed Feb 12 19:22:52 EST 1997


        Reply to:   RE>>A clean machine -Reply
Sorry group,
I should have included in my last message that we use 0.5N NaOH.
Happy flowing!
Florence

--------------------------------------
Date: 2/12/97 3:38 PM
To: cyto-inbox
From: Maryalice Stetler-Stevenson
What is the exact concentration of NaOH?

>        Reply to:   RE>>A clean machine -Reply
>We've been using a dilute sodium hydroxide solution to clean the tubing in
>our three
>FACscans for several years now.  We used to use bleach, as recommended by the
>manufacturer, and sometimes we would have clogs which never seemed to clear up
>completely.  Our protein chemist recommended NaOH to me late one afternoon when
>I was particularly frustrated with a stubborn clog.  The NaOH cleared it right
>out, and from then on we've used it regularly to keep the lines clear with few
>problems.  I've always wondered if anyone else has tried it and how it has
>worked for them.
>Florence
>
>--------------------------------------
>Date: 2/11/97 4:21 PM
>To: cyto-inbox
>From: Jim Zanghi
>
>>Small beads staying in the instrument can be a problem.  Triton X-100 at
>>0.1% (the same concentration used to permeabilize cells) in water will
>>remove most of them.
>>
>>Betsy Robertson
>>>
>>> Running ethanol
>>> after the beads seems to help push them through more quickly.
>>>
>>> Tony Bakke
>
>I was wondering about what types of solutions can be run through a flow
>cytometer without causing damage or corrosion.   Is pure ethanol okay?   On
>the advise of others, I  have run 50% hot bleach to clear up clogs, but I
>was very weary about doing this since bleach corrodes stainless steel.
>I've always followed with a 5 minute water rinse, but this concentration of
>bleach seems a bit extreme (we routinely use 10% bleach at room temp).
>If 0.1% triton or 70% ethanol is as effective as 50% hot bleach, than this
>seems to be the way to go.   Any thoughts about this?  What do
>manufacturers recommend?
>
>Jim
>
>--
>James A. Zanghi
>Dept. of Chemical Engineering
>Northwestern University
>Evanston, IL
>
>
>
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>Subject: Re: A clean machine -Reply

Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH



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Subject: Re: A clean machine -Reply






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