A clean machine -Reply

David L. Haviland, Ph.D. dhavilan at imm2.imm.uth.tmc.edu
Tue Feb 11 16:02:39 EST 1997


At 10:19 2/11/97 -0600, Jim Zanghi wrote:
>I was wondering about what types of solutions can be run through a flow
>cytometer without causing damage or corrosion.   Is pure ethanol okay?   On
>the advise of others, I  have run 50% hot bleach to clear up clogs, but I
>was very weary about doing this since bleach corrodes stainless steel.
>I've always followed with a 5 minute water rinse, but this concentration of
>bleach seems a bit extreme (we routinely use 10% bleach at room temp).
>If 0.1% triton or 70% ethanol is as effective as 50% hot bleach, than this
>seems to be the way to go.   Any thoughts about this?  What do
>manufacturers recommend?


I have the company recommendation for small quantities of bleach to be
taken through the sample tubing (as if it was a sample).   Then, I have
used 1% SDS 3 mls or so and then DDH20, where if the machine allows, I turn
it off with DDH20 in place (Calibur).    For an Elite, I had a technician
who also applied Jet-Dry to the sample tubing and nozzle and I never once
doubted her in the claim of  keeping salts from developing on the nozzle.
(She used, bleach, jet-dry, and DDH20 in that order prior to shut-down.)

As long a filters are by-passed, EtOH should be OK in pure analytical
machines.  It is not recommended for any model of sorter.  Despite any sort
of checklists, charged flow plates will often be left on and EtOH is the
*last* thing you want between charged plates as it a major fire hazzard.

As far a clogs go mid-run, I always keep that tube of 1-2% SDS handy.

David

=============================
 David L. Haviland, Ph.D.
 Asst. Prof. Immunology 
 University of Texas - Houston, H.S.C.
 Institute of Molecular Medicine  
 2121 W. Holcombe Blvd.  
 Houston, TX  77030 
 Internet:"dhavilan at imm2.imm.uth.tmc.edu" 
 Voice: 713.500.2413  FAX: 713.500.2424
 ------------------------------------------------------  
" Sometimes you're the windsheild, sometimes you're the bug."
=============================




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