A clean machine -Reply
Betsy R. Robertson
brrob at ims.alaska.edu
Mon Feb 10 12:21:44 EST 1997
Small beads staying in the instrument can be a problem. Triton X-100 at
0.1% (the same concentration used to permeabilize cells) in water will
remove most of them.
Betsy R. Robertson (907)474-7709 - Voice
Institute of Marine Science 474-7204 - FAX
University of Alaska Fairbanks
Fairbanks, Alaska 99775-1080 brrob at ims.alaska.edu
On Fri, 7 Feb 1997, Antony Bakke wrote:
> Nona Sheila R. Agawin asked:
> " is it always very hard to clean the flow cytometer after passing samples with
> fluorescent beads ?"
> I have found that small beads (< 4 micron) can stay in the instrument for a long
> time appearing in multiple samples after the beads were run. Running ethanol
> after the beads seems to help push them through more quickly.
> Tony Bakke
More information about the Cytometry