A clean machine -Reply

Betsy R. Robertson brrob at ims.alaska.edu
Mon Feb 10 12:21:44 EST 1997


Small beads staying in the instrument can be a problem.  Triton X-100 at 
0.1% (the same concentration used to permeabilize cells) in water will 
remove most of them.

Betsy Robertson
=========================================================
 Betsy R. Robertson               (907)474-7709 - Voice
 Institute of Marine Science           474-7204 - FAX
 University of Alaska Fairbanks
 Fairbanks, Alaska 99775-1080       brrob at ims.alaska.edu
=========================================================

 

On Fri, 7 Feb 1997, Antony Bakke wrote:

> 
> Nona Sheila R. Agawin asked:
> " is it always very hard to clean the flow cytometer after passing samples with
> fluorescent beads ?"
> 
> I have found that small beads (< 4 micron) can stay in the instrument for a long
> time appearing in multiple samples after the beads were run.  Running ethanol
> after the beads seems to help push them through more quickly.
> 
> Tony Bakke 
> 
> 
> 



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