Autofluorescence Quenching

Gerhard Nebe-von-Caron Gerhard.Nebe-von-Caron at
Mon Feb 10 07:06:58 EST 1997

          When browsing through my database I came across the 
          following hit upon which I remembered your recent inquiry.

Title:  Background-reducing compounds for probe-mediated in-situ fluorimetric 
Author: Cubbage, Michael Lee; Ju, Shyh Chen; Prashad, Nagindra; Weber, William 
Dugald; Bresser, Joel
Source: PCT Int. Appl. 95 19450 48 pp.
Year:   1995
Where Held:     
Info Category:  SUCL15
Company:        Aprogenex, Inc.USA 
Keywords:       in situ fluorometry background reducing dye:fluorescent staining
background reducing dye:hybridization fluorescent in situ background redn:
Issue:  9508    
Document Type:  
Language:       Eng
Assays for target mols. (e.g., viral nucleic acids, human genes, cellular or 
viral antigens) in biol. cells and viruses are disclosed wherein the use of 
appropriate light-absorbing mols. (e.g., Evans Blue, sulforhodamine 101, trypan 
blue, etc.) at an appropriate stage of the assay procedure leads to decreased 
nonspecific emission of light and/or decreased autofluorescence.

Gerhard Nebe-v.Caron
Unilever Research, Colworth,
Sharnbrook, Bedfordshire
GB - MK44 1LQ
Tel:    +44(0)1234-222066
FAX:    +44(0)1234-222344
gerhard.nebe-von-caron at

______________________________ Reply Separator _________________________________
Subject: Autofluorescence Quenching
Author:  wlauzon at at INTERNET
Date:    23/01/97 00:34


I've been lurking around this E-mail list for about a month now enjoying
the discussions and the information being exchanged.

I have recently heard of a technique used in flow based in situ PCR
where the autofluorescence is greatly reduced by the addition of trypan
blue.  It was just a casual reference in a talk and I was unable to find
anything more about it from the speaker.

I was wondering if there was anyone out there with experience in this
technique who could provide a protocol or a good reference for one.

While I have your ear, I would also appreciate any information that you
may have concerning epitope tagging reagents: anti-CDx-DIG/anti-DIG-PE
etc.  If there are commercially available reagents I would be eager to
find out the sources.

Thank you for your time

Wallace Lauzon
Dept. Microbiology and Immunology
University of Ottawa
451 Smyth Rd
Ottawa, Canada

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