anti-FLAG mAb intracellular staining...

Matthias Haury mhaury at
Mon Feb 10 07:55:55 EST 1997


I was wondering if somebody has experience detecting fusionproteins with a
"flag" epitope, using a commercial available anti-flag antibody for
intracellular expression...

I haven't tried it, but would like to know if this could possibly work,
especially for somebody who want's to analyse cellcycle expression of a
transfected protein using a flag-fusion construct.

Any details on which mAb to use, minimal expression levels, staining
procedures would be very wellcome.

Thank you very much,


Matthias Haury       Flowcytometry      Dept Immunology      Institut Pasteur
mhaury at      Tel: 33 (01) 40 61 31 29      Fax: 33 (01) 45 68 86 39

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