anti-FLAG mAb intracellular staining...

Matthias Haury mhaury at pasteur.fr
Mon Feb 10 07:55:55 EST 1997


Hello,

I was wondering if somebody has experience detecting fusionproteins with a
"flag" epitope, using a commercial available anti-flag antibody for
intracellular expression...

I haven't tried it, but would like to know if this could possibly work,
especially for somebody who want's to analyse cellcycle expression of a
transfected protein using a flag-fusion construct.

Any details on which mAb to use, minimal expression levels, staining
procedures would be very wellcome.

Thank you very much,

Matthias

_____________________________________________________________________________
Matthias Haury       Flowcytometry      Dept Immunology      Institut Pasteur
mhaury at pasteur.fr      Tel: 33 (01) 40 61 31 29      Fax: 33 (01) 45 68 86 39
_____________________________________________________________________________





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