Cytometry Course/Krakow

Jurek Dobrucki DOBRUCKI at mol.uj.edu.pl
Fri Feb 7 17:35:38 EST 1997





                Invitation and First Announcement

                  CYTOMETRY COURSE and WORKSHOP


        ----------------------------------------------------
        CONFOCAL IMAGING, FLOW CYTOMETRY, AND IMAGE ANALYSIS 
        ----------------------------------------------------


                     Krakow, 10-13 June 1997

  Sponsored by Bio-Rad Company and the Jagiellonian University

=================================================================
For detailed information about this Course, please check: 
               http://www.if.uj.edu.pl/~ubdobruc    or
               http://helios.mol.uj.edu.pl
On-line registration:
               http://helios.mol.uj.edu.pl/kurs/app.html
==================================================================

* PLACE: 
-------
    Jagiellonian University, Institute of Molecular Biology,      
    Krakow, Poland

* LANGUAGE: English
----------

* GENERAL OUTLINE: 
-----------------
     The Course and Workshop will embrace basic and some selected
     specialized techniques in:
               - confocal imaging
               - flow cytometry
               - image analysis

     There will be:
          LECTURES - open to all, no fee
          PRACTICALS/TUTORIALS (experiments and discussion)

* TUTORS and LECTURERS:
-----------------------
Prof. G.J.Brakenhoff, University of Amsterdam, The Netherlands
Prof. Z.Darzynkiewicz, New York Medical College, USA
Prof. P.Robinson, Purdue Cytometry Laboratories, Purdue, USA
Dr J.V.Watson, Addenbrooke's Hospital, Cambridge, UK
Dr N.S.White, Department of Plant Sciences, Oxford University, UK


* PARTICIPANTS:
---------------
The Course is aimed at graduate students and young researchers from
areas of biology, biotechnology, medicine, chemistry, physics.

Participants are welcome to attend the lectures only (free of
charge) or register for practicals (tuition fee).


* LECTURES:
----------- 
The lectures will cover:

        CONFOCAL:
               Principles of Confocal Microscopy
               Choice of chromophores, laser lines, filters, lenses
                    in confocal microscopy
               Photon statistics, sampling rate, confocal pinhole
                    - optimization of data collection
               3-D image collection, restoration and analysis
               Use of high NA oil and water immersion lenses
               Resolution and contrast in confocal imaging

        FLOW:
               Principles of Flow Cytometry                 
               Basic parameters: fluorescence, light scatter
               Basic immunophenotyping using multicolour analysis 
               Cell cycle analysis, viability and apoptosis by flow
                    cytometry  
               Measurement of oxidative metabolism and cell
                    function
               Flow measurements of platelet activation 
                 
        IMAGE ANALYSIS:
               Basic 2D image analysis 
               Image manipulation - noise, contrast, edge
                    enhancement, deconvolution


* PRACTICALS
------------
Practicals will cover:

        CONFOCAL
               Image acquisition - basic principles 
               Optimizing fluorescent image acquisition: role of
                       a confocal pinhole, sampling rate, etc.  
               Reflected light imaging
               Multicolour image acquisition - bleedthrough
                    correction
               Acquisition of 3D data sets

               * Additionally: 
                    We will offer a special tutorial on
                    microinjection of cells in culture

        FLOW
               DNA cell cycle analysis  
               Cell function by flow cytometry      
               Phenotyping  
               Multiparameter Data Analysis 
               Platelet activation
               
        IMAGE ANALYSIS
               Basic 2D image analysis 
               Image manipulation - noise, contrast, edge
                    enhancement
               3-D image restoration and analysis
               Deconvolution of confocal and wide field
                    fluorescence images

Participants of practicals/tutorials will gain hands-on experience
using:
              
               >> a Bio-Rad MRC1024 confocal microscope
               >> three Silicon Graphics workstations
               >> BioRad, Beckton-Dickinson and Coulter flow
                  cytometers

     The Course participants will study live and fixed
     animal and plant cells. 

Suggest your own topic for a tutorial
=====================================
The prospective students are encouraged to suggest subjects of
practicals that they will find most useful (refer to the
Application Form).

Bring your own samples
=====================
The participants are welcome to bring their own samples to be
studied during practicals (no clinical material, please). 

Please note:
        Enrollment for practicals is limited to 20 students


* TUITION FEE and DEADLINES
---------------------------
Deadline for applications is March 15. 

The tuition fee, payable to the Jagiellonian University, is:
     $100,-  for graduate students,
     $150, - for faculty members
- if received before March 15, 1997

The tuition fees received after March 15 will be $150 and $200,
respectively.

The tuition fee includes the course materials, handouts, a ticket
for a banquet. Hotel, meals, etc. are not included.

Please transfer the tuition fee to the following account:

        Bank Przemyslowo-Handlowy, Oddzial IV Krakow,
        Account no.: 10601389-1023-27000-400101

        Please do _not_ _forget_ to include a note "Confocal      
        Imaging Workshop"!


* FINANCIAL SUPPORT, STIPENDS
-----------------------------
Financial support will be available to participants who registered
for the Course. Please contact dr Dobrucki for details.


* FREE CONFOCAL (http://helios.mol.uj.edu.pl/kurs.html#free)
------------------------------------------------------------
All students who successfully completed practicals on confocal
microscopy will be given 15 hours of use of the BioRad MRC1024
confocal microscope at the Jagiellonian University - free of
charge. These students will be welcome to continue their research
projects immediately following the Course or return at a later
date. 

* ACCOMMODATION
---------------
A number of reasonably priced ($25-50//night) rooms have been
booked for the Faculty and Students at the Guest Houses of the
Jagiellonian University. Advance booking is required. Please
contact dr Dobrucki for details.

Please note:
June is a high tourist season in Krakow and only the pricey hotels
may have vacancies! Moreover, the Course coincides with a visit of
Pope John Paul II in Krakow. Early booking for University Guest
Houses is strongly recommended. 


* COURSE CERTIFICATES
---------------------
Those who attended all Course lectures and successfully completed
at least four selected practicals\tutorials will be entitled to
receive a Course Certificate.


* REGISTRATION
--------------
Register on-line via our WWW page:
        http://helios.mol.uj.edu.pl/kurs/app.html

or use the application form attached at the end of this document


* SIGHTSEEING AND SOCIAL PROGRAMME (more on our WWW page)
---------------------------------------------------------
The following trips are planned:

        Museum of the Jagiellonian University (Collegium Maius) 
          featuring a unique collection of scientific equipment,
          including old microscopes, copies of astronomical
          instruments used by Nicolaus Copernicus, and more.
        The medieval Salt Mine at Wieliczka
        The Tatra Mountains
        Krakow Old Town and the Royal Castle of Wawel

We are also planning a trip to a Museum at Auschwitz-Birkenau
(Oswiecim-Brzezinka) concentration camps.

* FURTHER INFORMATION:
---------------------
        dr Jerzy Dobrucki
        dobrucki at mol.uj.edu.pl
        http://www.if.uj.edu.pl/~ubdobruc
        fax.   48 12 / 33 69 07
        tel.   48 12 / 34-14-42, ex. 268
               48 12 / 34-16-80, ex. 268
address: 
        Jagiellonian University
        Institute of Molecular Biology
        Al. Mickiewicza 3
        31-120 Krakow
================================================================
                            APPLICATION FORM

        CONFOCAL IMAGING, FLOW CYTOMETRY, AND IMAGE ANALYSIS

                            Krakow, 10-13 June 1997

Please type in using CAPITAL LETTERS

First Name:.................................
Family Name:.................................
Occupation (undergraduate, graduate, post-doc, faculty,
other):.................
Institution: 
        full name............................
        postal address:........................
        telephone...............................
        fax.....................................
        electronic mail.........................

Name and address of your supervisor, group leader or laboratory
director:
        ....................
        ....................
        ....................
        ....................
        ....................

Prime interest (please mark with X): 
        ... confocal imaging and image analysis
        ... flow cytometry

I want to attend (please mark with X):
        ... lectures only (free of charge)
        ... practicals and lectures (I will transfer the tuition
               fee)

I suggest the following topics for tutorials/practicals:
1...................
2...................
3...................
4...................

        
Please enclose your curriculum vitae, including a list of
publications
                            
---------------------------------------
Please return the form and cv by mail, fax or e-mail to:

dr J.Dobrucki
Jagiellonian University
Institute of Molecular Biology
Department of Biophysics
Al. Mickiewicza 3
31-120 Krakow
Poland

dobrucki at mol.uj.edu.pl
fax.  48 12 / 33 69 07
=======================================================
J.Dobrucki
Laboratory of Confocal Microscopy and Image Analysis
Department of Biophysics
Jagiellonian University
Al. Mickiewicza 3
31-120 Krakow
Poland
    tel. (48-12) 34-14-42, ex. 268
    fax. (48-12) 33-69-07
    Internet: dobrucki at mol.uj.edu.pl
    WEB:      http:/www.if.uj.edu.pl/~ubdobruc
=======================================================



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