Request to Join CYTOMETRY from the web server

Abby Allen allen at cbr.med.harvard.edu
Thu Feb 6 14:40:44 EST 1997


kevinnsara at sprintmail.com wrote:
> 
> I'm looking for a procedure to evaluate phagocytosis by flow cytometry on dogs and cats.  Help!!?


Coulter is coming out with (came out?) some reagents which may help
you.  Their new product line is known as the Cell Probe Reagents.  One
of these reagents (as I understand it) is e-coli labeled with PE-Cy5 (I
think).  They have a number of other reagents, one of which measures
oxidative burst.  This reagent is rhodamine labeled.  I was told that I
could use these reagents in parallel to measure phagocytosis in humans. 
I don't know if they work in dogs and/or cats.  I have a lot of
experience using the reagent for oxidative burst (it works well), but I
have not used the e-coli reagent.  

I hope this helps you!

Abby Allen
Center for Blood Research
Boston, MA  02115
allen at cbr.med.harvard.edu



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