RNA and FACS

julia philips jphilips at mail.usyd.edu.au
Mon Feb 3 23:39:21 EST 1997


Hello all,

I've just been approached by someone wishing to look at RNA expression using
flow cytometry. He wants to do 2-colour analysis if possible and already has
his probes made up. What would be the best approach (We're using a FACScan -
488nm) ? I think his probes are DIG-labelled, so we might try to use an
anti-DIG antibody labelled with FITC or PE. 
Does anyone have any basic protocols or ideas that we might be able to use,
as RNA probes and analysis by FACS is a new and exciting approach over here.

Thanks in advance

Julia Philips

**************************************************
Julia Philips
Institute of Dental Research
2 Chalmers St
Surry Hills
NSW 2010
Australia
PH: 61-2-9282 0358
FAX: 61-2-9282 0368
email: 	jphilips at extro.ucc.su.oz.au
**************************************************




More information about the Cytometry mailing list