comparison annexin V versus TUNEL results

Sabido Odile Odile.Sabido at
Thu Dec 18 08:54:51 EST 1997


I have a problem concerning the interpretation of annexin V positive cells
analysed by CMF.

The cells are not trypsinized, but released from the tissue by collagenase
digestion, which probably does not damage the cell membrane.

How can we explain the increase, with the culture time, of the percentage
of annexin V positive cells (reaching 78%), whereas TUNEL method gives
always negative results (TUNEL used as a routine method in our laboratory
on other cell types) ?

Thank you for any help !

Odile Sabido
Faculte de medecine Jacques Lisfranc
Centre Commun de Cytometrie en Flux
15, rue Ambroise Pare
42023 Saint-Etienne cedex 02.
Tel : 33 (0) 4 77 42 14 41
Fax : 33 (0) 4 77 42 14 89
sabido at
web site

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