sticky antibody

Julie Stevenson Julie.Stevenson at unsw.edu.au
Tue Aug 19 11:57:49 EST 1997


We are currently using a Pharmingen anti-CD99 antibody to stain a CD34+
cells from cord blood. However, it seems to be causing the cells to clump,
while the same cells, stained with different antibodies are fine. It's a
mouse IgG2a. Does anybody have any idea about why this is happening, or how
we could stop it, as it is causing us to lose large numbers of precious cells.

Thanks in advance,
Julie

_____________________________________________
Julie Stevenson

Bone Marrow Transplant Lab,
Children's Cancer Institute of Australia,
Sydney Children's Hospital
Randwick, NSW, 2031

Ph 61 2 9382 1819, Fax 61 2 9382 1850
email julie.stevenson at unsw.edu.au
______________________________________________






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