FW: DNA stain

Ross Longley Longley at HBOI.edu
Mon Apr 14 07:25:52 EST 1997


You might try using SYBR Green I which can be excited by blue (488)
light.  We have begun to look picoplankton samples in marine samples and
this stain seems so far, to do the job.  The reference for the technique
is:

"Enumeration of cell cycle analysis of natural populations of marine
picoplankton by flow cytometry using the nucleic acid stain SYBR Green I
by Dominique Marie, Frederic Partensky, Stephan Jacquet and Daniel
Vaulot, Applied and Environmental Microbiology, Jan 1997, Vol 63, Nol 1,
p 186-193."

Ross Longley

------------------------------------------------------------------------
-----------------------------------------
Ross E. Longley, Ph.D.
Immunology and Oncology Group Leader
Division of Biomedical Marine Research
Harbor Branch Oceanographic Inst.
5600 U. S. #1, North
Fort Pierce, FL  34946
(561) 465-2400, Ext 486
(561) 465-1523, FAX
longley at hboi.edu
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>----------
>From: 	agawin at azathoth.ceab.es[SMTP:agawin at azathoth.ceab.es]
>Sent: 	Thursday, April 10, 1997 11:58 PM
>To: 	Cytometry Mailing List
>Subject: 	DNA stain
>
>
>Dear Cytometers,
>
>
>I am looking for DNA stain to do cell cycle analysis for natural
>picoplankton populations but our cytometer restricts me from using
>UV-excited dyes. I hope somebody can help me out.
>
>Thanks in advance,
>
>Nona Agawin
>
>Nona Sheila R. Agawin
>Centro de Estudios Avanzados de Blanes (CEAB)
>Consejo Superior Investigaciones Cientificas (CSIC)
>Cami de Sta. Barbara s/n
>17300 Blanes, Girona
>Spain
>FAX: 34 72 337806
>Phone: 34 72 336101
>WWW page: http//www.ceab.es
>
>
>
>



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