BarrenP at MedImmune.com
Fri Apr 4 10:00:56 EST 1997
Does anyone have any experience with sorting bacteria (Streptococcus)
on a stream in air flow cytometer. I am using a
What I think I need is a dye that will allow me to counter stain all
the bacteria (preferably live) to be used in conjunction with a dim
surface label, and then sort on the surface labeled positives. The
counter stain would need to be dim so as not to interfere with the dim
I have tried to sort with a SSC log threshold. and this does not work.
As soon as I vibrate the stream I loose all low end resolution which
is where my bacteria are.
I need to use a fluorescent trigger to threshold on, this would be less
sensitive to stream vibrations which is what I assume is killing me on
the log SSC threshold.
Just a note all my Scatter parameters are on logarithmic amplification.
Thanks in advance for your time and energy
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