Fixation of FDG ?

Martin Poot Martin at probes.MHS.CompuServe.COM
Mon Nov 25 17:37:40 EST 1996


Dear Dr. Haury,

We found that fluorescence after CM-FDG staining for B-gal activity 
was quite well retained after 3.7% formaldehyde fixation.  We presume 
that the chloromethyl moiety of CM-FDG reacts with protein thiols.  It 
should be noted that some of the fluorescent products of CM-FDG, and 
also FDG, get lost from cells.  With verapamil one can prevent that. 
We have submitted a paper describing all this.

Hope this helps,

Martin Poot

Martin_Poot at probes.com







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