cell cycle analysis with PI

Albert D Donnenberg, PhD donnal at novell1.dept-med.pitt.edu
Tue Nov 19 17:38:38 EST 1996


Flow'ers
We have started a project looking at intracellular proteins (p21, 
p27) in combination with DNA content.  Proteins are measured by 
indirect fluorescence (FITC GAM) and DNA with PI.

Although our early data is interpretable, we have noticed that the PI 
cvs vary and are not always as sharp as we would like.

What factors most affect the tightness of DNA peaks measured with PI?

Your help is most appreciated,


*******************************
Albert D. Donnenberg, PhD
Associate Professor of Medicine
University of Pittsburgh
W1056 BST
200 Lothrop St.
Pittsburgh, PA 15213
(412) 624-9596
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