Use of Acridine Orange in killing assay.
Surgical Labs Resident
SURGLAB1 at is.rvh.mcgill.ca
Mon Nov 18 12:30:00 EST 1996
This is my first time using "the list" and I think it's great,
especially since I am a newcomer to the world of flow cytometry. I'm trying
to adapt an old microscopic technique that uses acridine orange in a
neutrophil killing assay, where candida or staph. a. is used as the target.
A.O. is supposed to passively enter the effector cells, and shift from green
to red fluorescence when phagocytosed targets are killed. Can anyone out
there explain this to me in a little more detail and tell me if it would
work with flow cytometry?
Royal Victoria Hospital
Montreal, Quebec, Canada
More information about the Cytometry