4th color options

Albert D Donnenberg, PhD donnal at novell1.dept-med.pitt.edu
Mon Nov 18 14:37:52 EST 1996


Continuing on the thread of 4 color combinations which work well with 
a single laser:

We are using FITC, PE, ECD (PE-Red610), and PE-Cy5 with good results.
This is the combination recommended by Coulter for use on the 4-color 
XL.  The only caveat (which probably goes for other 4-color 
combinations as well):  it is important to titrate the reagents and 
become familiar with their single color staining properties before 
using them together.
*******************************
Albert D. Donnenberg, PhD
Associate Professor of Medicine
University of Pittsburgh
W1056 BST
200 Lothrop St.
Pittsburgh, PA 15213
(412) 624-9596
*******************************



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