4th color options

Albert D Donnenberg, PhD donnal at novell1.dept-med.pitt.edu
Mon Nov 18 14:37:52 EST 1996

Continuing on the thread of 4 color combinations which work well with 
a single laser:

We are using FITC, PE, ECD (PE-Red610), and PE-Cy5 with good results.
This is the combination recommended by Coulter for use on the 4-color 
XL.  The only caveat (which probably goes for other 4-color 
combinations as well):  it is important to titrate the reagents and 
become familiar with their single color staining properties before 
using them together.
Albert D. Donnenberg, PhD
Associate Professor of Medicine
University of Pittsburgh
W1056 BST
200 Lothrop St.
Pittsburgh, PA 15213
(412) 624-9596

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