Cryo Preserved Cell Staining

Scott R Burger burge009 at
Fri Nov 15 16:52:53 EST 1996

>Anyone with any experience in thawing bags of frozen marrow and keeping 
>things alive?
>need help
>jim houston

Sure!  We do this in the clinical setting for autologous marrow transplants.
We also do this to obtain hematopoietic progenitor cells and endothelial
cells for more basic research.  

Can you describe a bit more specifically what you have in mind?  Are there
specific cell populations you want to culture -- stem and progenitor cells,
stromal cells, endothelial cells?  How long do want to keep the cells alive?
What will you be doing with the cells?


Scott R. Burger, M.D.
Medical Director, Cell Processing Laboratory
University of Minnesota Hospital and Clinic
burge009 at
612-624-5411 (Fax)

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