Absolute counts from flow cytometry
douglas at helix.nih.gov
Fri Nov 15 07:38:36 EST 1996
At 04:29 PM 11/14/96 EST, you wrote:
>We are trying to generate absolute counts for different cell types in the
>peripheral blood of mice. What are your preferred methods? ......
Human Genome Sciences
You may want to look at LDS-751. It is a nucleic acid dye not requiring
permeabilization and can be used in whole blood with no lysing.
See the following reference:
Multidimensional Flow Cytometric Blood Cell Differentiation without
erythrocyte lysing, Terstappen, et al. in Blood Cells (1991) 17:585-602.
The dye is available from Exciton, Inc (513) 258-3937, Dayton, Ohio.
More information about the Cytometry