Absolute counts from flow cytometry

Jeff Carrell Jeff_Carrell at hgsi.com
Thu Nov 14 16:29:15 EST 1996

We are trying to generate absolute counts for different cell types in the
peripheral blood of mice.  What are your preferred methods?

I understand that one common practice is to multiply the WBC (from an automated
cell counter such as a Coulter Counter) by the percent staining (from FACS). 
However, we are questioning the validity of this approach because of the
different lysing methods used for the two types of instruments. Some non-
erythrocytes may be lysed in different proportions with each method.  Is
multiplying this WBC count by a relative percentage accurate?  

Of course, these instruments were designed for human blood, so trying to adapt
procedures for mice is a challenge...but it shouldn't be impossible.  Any
thoughts would be greatly appreciated.

Jeff Carrell
Human Genome Sciences

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