Krypton Laser for GFP?

Pizzo,Eugene Pizzo at nso1.uchc.edu
Wed Nov 13 07:33:00 EST 1996



All Colleagues,

A researcher here at UCONN is considering using the 413nm violet line
of a Krypton laser to excite mutant GFP.  This researcher has been
somewhat dissatisfied with our results using 488nm excitation on the
FACStar Plus (by the way we've been using a 500 EFLP in Fl1 to
capture the emission).  Can anyone enlighten me as to the worthiness
of this approach?  Is there a new GFP out there or is 413nm excitation
really significantly better?

Gene Pizzo/UCONN Health




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