Glutaraldehyde

Martin Poot Martin at probes.MHS.CompuServe.COM
Tue Nov 12 17:01:14 EST 1996


Dear Flow-ers,

I response to the question of Jan Keij as to how to block a 
glutaraldehyde fixation.  What about using Tris ?  It has free amines 
which react avidly with all aldehydes.  Just replace your phosphate 
buffer with a Tris buffer and your glutaraldehyde will become 
"neutralized" in a single wash step.

Molecular Probes, Inc. does not offer Tris for sale nor has any 
commercial interests in Tris (in case you would wonder).

Martin Poot



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