CMV-specific surface antigens

SPERFETTO sperfetto at
Tue Nov 12 10:02:27 EST 1996


Actually,  Skip Maino from BD has described a antigen specific proliferation 
test that works in HIV.  Patient's PBMC are cultured with and without CMV 
extract.  After culture the cells can be measured for CD69.  They showed that a 
CMV+ donor would have 10%+ CD69 cells while a CMV sero negative donor would have 
only 3% CD69 cells.  This might be a better approach than quantitating the 
actual AG on PBMC.

Stephen P. Perfetto
Department of HIV Disease Prevention
Walter Reed Army Institute of Research
1600 East Gude Drive
Rockville, MD. 20850
Subject: CMV-specific surface antigens
From:    "Gregor Rothe" <gregor.rothe at> at 
Date:    11/8/96  2:23 PM

Hello to all,

Is there anyone on the list with experience in the flow 
cytometric analysis of CMV-specific nuclear or cell-
surface antigens on peripheral blood leukocytes?
Any recommendations regarding reagents?

Thanks for any help,

Gregor Rothe
Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, Germany

Tel. +49 (941) 944-6204
Fax  +49 (941) 944-6202

Internet: Gregor.Rothe at

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