CMV-specific surface antigens

SPERFETTO sperfetto at hiv.hjf.org
Tue Nov 12 10:02:27 EST 1996


Gregor

Actually,  Skip Maino from BD has described a antigen specific proliferation 
test that works in HIV.  Patient's PBMC are cultured with and without CMV 
extract.  After culture the cells can be measured for CD69.  They showed that a 
CMV+ donor would have 10%+ CD69 cells while a CMV sero negative donor would have 
only 3% CD69 cells.  This might be a better approach than quantitating the 
actual AG on PBMC.

Stephen P. Perfetto
Department of HIV Disease Prevention
Walter Reed Army Institute of Research
1600 East Gude Drive
Rockville, MD. 20850
_______________________________________________________________________________
Subject: CMV-specific surface antigens
From:    "Gregor Rothe" <gregor.rothe at klinik.uni-regensburg.de> at 
Internet_Gateway
Date:    11/8/96  2:23 PM


Hello to all,

Is there anyone on the list with experience in the flow 
cytometric analysis of CMV-specific nuclear or cell-
surface antigens on peripheral blood leukocytes?
Any recommendations regarding reagents?

Thanks for any help,

Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, Germany

Tel. +49 (941) 944-6204
Fax  +49 (941) 944-6202

Internet: Gregor.Rothe at klinik.uni-regensburg.de
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