Cell Cycle Software

Terry Hoy Hoy at cardiff.ac.uk
Mon Nov 11 05:44:02 EST 1996

Austin Lin enquired :
>I need some advices regarding the use of ModFit S/W.
>For example, one set of data can 
>have 10% G0/G1, 80% S and 10% G2/M.  The s/w scans three peaks and take the 
>second S peak as G2/M peak. Another example will be a set of data with 20% 
>G0/G1 and 80% S and invisible G2/M. The s/w will scan and see only one 
>Would anyone suggest a good 
>Mac-s/w for us? (We have a FACScan and Macintosh Quadra 650) Thank you in 

I have recently processed several hundred histograms with 'cylchred'. 
Some of these have been BrdU +ve/-ve fractions following pulse 
labelling. Many of these fit the description above and others have 
been more extreme. The program will attempt to find G0/1 G2/M 
positions, if you are not happy with these positions values can be 
called back from the previous histogram or inserted manually.
Calculation of phases is then based on Watson et al Cytometry 8 1-8 
which handles synchronised populations as well as more conventional 
Version 1.0.5 uses wildcards for easy selection of groups of files 
and produces a textfile of results for transferring into 
spreadsheets. It is PC based but I have seen it operating on a Mac 
with SoftWindows.

Copies from
That takes you to my home page which starts with an 
illustration from Cylchred 
Follow the links to 'Software from Cardiff'

If you prefer ftp
OPEN    ftp.cf.ac.uk
CD   /pub/UWCM/hg
GET cylchred.exe (as binary).


Dr. Terry Hoy
Principal Research Officer
Department of Haematology                   Telephone 44 1222 743458
University of Wales College of Medicine           FAX 44 1222 744655
Heath Park                                  E-mail hoy at cardiff.ac.uk
CARDIFF CF4 4XN           http://www.cf.ac.uk/uwcm/hg/hoy/index.html

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