Cellquest to PC

Pizzo,Eugene Pizzo at nso1.uchc.edu
Fri Nov 8 10:10:16 EST 1996

All colleagues,

      Several individuals were interested in the response I received to my 
question about
translating PC acquired data to the Mac following our upgrade of our FACScan 
purchase of a FACSCalibur.

Let me first thank everyone who generously responded. It was a considerable 
help to
me.  Nan Harvey and Matthias Haury gave especially detailed and extensive 
which were particularly helpful, Thanks!  But they should not be held 
responsible if I fail to
relate or interpret there advice accurately.

We finally chose the easiest route, which was to put ethernet cards in all 
the machines and transfer all data over the network utilizing a shared drive 
between our PC's and Mac's.   Allow me just this; as a long time only PC 
user I am convinced that you can go from PC to Mac but you would never go 
the other way.  What marvelous machines these Mac's!  Not only are they 
completely intuitive to run but even the engineering of the box is 
remarkably well done, for changing boards, making upgrades etc.  Our shared 
drive on the PC shows up as a  new drive letter in characteristic hfs format 
to which you can copy files but on the Mac its this cute little icon we 
named 'cytometry'  that you just drag your files to or more likely entire 
folders and drop them in - I love it!  These Cellquest acquired files then 
show up on the PC and can be analyzed in PC-LYSYS or WinMDI.  It's great!! 
 but as predicted it hasn't taken long for users to appreciate the power of 
CellQuest over LYSYS II and simply do there analysis on the Mac.

PC formatted floppies containing FCS 2.0 data files can be run on the Mac 
using CellQuest simply
by importing them into FacsConvert which came with our FACSCalibur. Although 
this software is
designed to convert 1.0 HP formatted files to 2.0 I have found that running 
FACSConvert on the Mac
effectively translates files from PC format to Mac format.  PC Exchange is 
supposed to do this but
I haven't figured out how to generically describe the suffix to enable PC 
Exchange to recognize
what software is meant to be utilized for their analysis.  I'm guessing that 
the same could be done
with files stored on a PC formatted Zip drive and placed in a Mac connected 
Zip Drive but I haven't
tried this yet.  To go the other way , Mac to PC, (instead of networking) 
Matthias Haury also pointed out that a PC formatted Zip disk in a Mac Zip 
Drive could be used to acquire CellQuest data on the Mac/PowerPC since the 
Mac will be able to write to these disks utilizing PC Exchange and obviously 
these disks could then be read directly by the PC's Zip Drive.

We were fortunate that our network people are allowing us use of this shared 
drive, alternatively I
suppose we would have had to ftp from one computer to the other using a 
program like Fetch and I haven't tried that as yet.

Gene Pizzo  (860)679-3463 UCONN Health

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