Dead Cell Marker on 633 nm FL4 ?

Matthias Haury mhaury at pasteur.fr
Thu Nov 7 11:56:49 EST 1996


Hello me again,

Those guys out there that have already experience with the FACScalibur 4
Colors (or equivalent setup on another machine), is there a dye that you
can use on the FL4 to exclude dead cells, while still using FITC, PE,
Tricolor for Surface markers ?

I remember to have heard something about TOPRO etc, but which works the best ?

Thanks for details (dye, manufacturer, compensations, staining concentrations).

Merci,

Matthias

______________________________________________________________
Matthias Haury                      _/_/_/    _/_/_/    _/_/_/
Flowcytometry - Immunology           _/     _/    _/  _/    _/
Institut Pasteur Paris              _/     _/_/_/    _/_/_/
Email: mhaury at pasteur.fr           _/     _/        _/
Tel:   33 (01) 40 61 31 29      _/_/_/   _/        _/
Fax:   33 (01) 45 68 86 39      INSTITUT PASTEUR   PARIS
______________________________________________________________





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