TUNEL - double staining

triss@promega.com triss at promega.com
Wed Nov 6 09:44:11 EST 1996


Regarding double staining with TUNEL and something else, here are a few 
references to get you started.

Qioa, L. et al. Flow cytometric characterization of proliferation 
associated nuclear antigen (p105) during cell cycle in normal lymphocytes 
and promyelocytic leukemia cells (HL-60). Analytical and Quantitative 
Cytology and Histology 17(3): 183-188, 1995.

Tornusciolo, DRZ et al. Simultaneous detection of TDT-mediated dUTP-biotin 
nick end-labeling (TUNWL)-positive cells and multiple immunohistochemical 
markers in a single tissue section. BioTechniques 19: 800-805, 1995.

Raza, A et al. Simultaneous assessment of cell kinetics and programmed cell 
death in bone marrow biopsies of myelodysplastics reveals extensive 
apoptosis as the probable basis for infective hematopoeisis. Am. J. 
Hematology 48: 143-154, 1995.

Mundle, S. et al. Novel insitu double labeling for simultaneous detection 
of proliferation and apoptosis. J. Histochem. Cytochem. 42(12): 1533-1537, 
1994.

I hope this helps.

Terry Riss


______________________________ Reply Separator _________________________________
Subject: TUNEL - double staining
Author:  grggta at picr.cr.man.ac.uk (Graham Atherton) at INTERNET-MAIL
Date:    11/5/96 4:51 PM




Is there a method which allows a fibroblast cell line to be stained up with 
an antibody for a cytoplasmic protein (not a surface marker) eg lacZ 
transfectedand then to 'stain up' for apoptotic nuclei via TUNEL or
similar so that one
may determine the number of lacZ +ve cells which are apoptotic?
All of the papers I have read so far describe methods for doing one or the 
other, not both - one in fact states that the two are incompatible under 
their experimental conditions.
Many thanks for any help
Graham Atherton

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Subject: TUNEL - double staining
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