Increasing Cytoplasmic fluorescence

Deborah Berglund umbbd at gemini.oscs.montana.edu
Wed Nov 6 14:15:28 EST 1996


Hi,

I used to see similar increases when staining with FDA, as it leaked out 
of the cells the medium surrounding the cells became fluorescent, 
therefore increasing the signals.

We timed the analyses precisely so that each sample was analyzed exactly 
5 minutes after staining.

Deb Berglund
Montana State University




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