cytoplasmic CD3 or cytoplasmic CD22

Elizabeth Paietta paietta at
Tue Nov 5 15:51:15 EST 1996

On Tue, 5 Nov 1996, Tim wrote:

> I would like to post the following message on the flow cytometry
> listserv:
> Does anyone routinely use cytoplasmic CD3 or cytoplasmic CD22 for acute
> leukemias?  If so, what has been your experience?  Are these markers
> helpful?  We are considering adding these to our panel.  
> Tim Singleton, MD
> singleto at
Hi, Tim, regarding your question on intracytoplasmic CD3 and CD22, I am 
routinely staining for these antigens in combination with 
myeloperoxidase.  We are using Caltag's FIX & PERM reagents for fixation 
and permeabilization and have good experience.

Elisabeth Paietta

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