TUNEL - double staining

Graham Atherton grggta at picr.cr.man.ac.uk
Tue Nov 5 05:24:19 EST 1996



Is there a method which allows a fibroblast cell line to be stained up with
an antibody for a cytoplasmic protein (not a surface marker) eg lacZ
transfectedand then to 'stain up' for apoptotic nuclei via TUNEL or
similar so that one
may determine the number of lacZ +ve cells which are apoptotic?
All of the papers I have read so far describe methods for doing one or the
other, not both - one in fact states that the two are incompatible under
their experimental conditions.
Many thanks for any help
Graham Atherton



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