hematagones vs ALL?

kwasi ricardo at icr.ac.uk
Thu Mar 28 12:20:22 EST 1996

Frederic Preffer wrote:

Does anyone have any immunological techniques of distinguishing hematagones
(TdT+ CD19+ CD10+), commonly found in reactive bone marrows,   from ALL??
When faced with this possiblity, we must "return" to morphology...something
I rather not have to admit to..

I agree with you that it is difficult to admit it, but now we have the solution.
We developed a method by flow cytometry to differentiate between them. It is based in the 
quantification of TdT and CD10 CD19 and it works so well that we have the clinicians ringing to 
get our results before reporting the bone marrows.
I am presenting a poster in Rimini about it.

The reference is:

Quantitative flow cytometry can distinguish between normal and leukaemic B-cell precursosrs.
N. Farahat et al. British Journal of Haematology, 1995, 91, 640-646

I hope it works in your hands.

Ricardo Morilla,
Academic Department of Haematology and Cytogenetics,
Royal Marsden Hospital,
Fulham Road,
London SW3 6JJ.
TEO 0171 352 8171.
Fax:0171 351 6420

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