isotype controls

Alice L. Givan Alice.L.Givan at Dartmouth.EDU
Tue Mar 26 09:43:00 EST 1996


Just to second Mario Roederer's sound comments on isotype controls -- and the
complications of needing to match control concentration with stain
concentration (when we often don't know the concentration of either....).

There is,  in addition,  the added complication if using directly conjugated 
antibodies.  For the isotype control to be valid,  it would also have to match
the staining antibody with respect to its F/P ratio.  And accurate
determination of F/P ratios for antibodies used in flow cytometry is a huge can
of worms.....
Alice Givan
Englert Cell Analysis Laboratory 
Dartmouth Medical School
Lebanon,  New Hampshire
USA



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