TUNEL Vagary

Christopher Froelich, M.D. granzyme at merle.acns.nwu.edu
Mon Mar 25 12:59:00 EST 1996


Hello folks

We've been using the FITC-dUTP variant of the TUNEL assay (paraformaldehyde
fixed cells stored in PBS) to study granzyme/perforin mediated apoptosis.
We've recently noted an unexpectedly high percentage of cells being stained
in our negative control populations (yielding a bi-modal populations).

There are only two recent changes:
1) a new lot of 4% paraformaldehyde
2) repeated freeze-thaw cycles of the FITC-dUTP stock.

any help would be greatly appreciated

Thanks in advance

Chris Froelich








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