lymphomas by flow -Reply

Karen Henell henellk at ohsu.edu
Sun Mar 24 17:50:25 EST 1996


Karen Mann asked about media and transport effects on viability of lymph
node and lymphoma cells on 3/19/96.

We have good luck maintaining viability of T and B lymphocytes in normal
whole or even tissue-embedded lymph nodes using Hanks Balanced Salt
Solution and wet ice for the first 12-24 hours post excision.  To improve
viability for longer storage or for more delicate cells our medium of
choice is RPMI 1640, again held at 4 degrees C.

Hope this helps.

Karen Henell
Histocompatibility Technologist
Laboratory of Immunogenetics and Transplantation
Oregon Health Sciences University
Portland, OR  97201  USA
henellk at ohsu.edu






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