Lymph Node Prep

Moss, Delynn M. dmm3 at CIDDPD2.EM.CDC.GOV
Fri Mar 22 10:07:00 EST 1996


We do not use separation medium on single cell suspensions from lymph nodes 
of
mice.  FCS and SSC profiles normally show a single population of lymphocytes 
only.
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From: owner-cyto-sendout
To: Cytometry Mailing List
Subject: Lymph Node Prep
Date: Tuesday, March 19, 1996 3:25AM

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From: "Keith Bahjat" <KBahjat at msn.com>
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To: Cytometry Mailing List <cytometry at flowcyt.cyto.purdue.edu>
Subject: Lymph Node Prep
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Can someone please tell me if there is any benefit to using density gradient 

separation on cells from a lymph node (be it a biopsy or FNA) if you are
already using viability gating?
What I saw with viability gating looked pretty straight forward, and I
couldn't see why any separation media would be needed.
I would be interested in any and all experience people have had with the two 

techniques.
Thanks in advance.

Keith Bahjat
South Bend Medical Foundation
South Bend, Indiana
219-234-4176 x282
E-mail: Kbahjat at MSN.com



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