quantifying mitochondria

maurice r.g. o'gorman mogorman at anima.nums.nwu.edu
Wed Mar 20 09:39:03 EST 1996


hello all hope your enjoying the first day of spring

i recently had an inquiry from a colleague on the possibility of measuring
(qualitative or relative measures are ok) mitochondria in fetal rat hind
muscle tissue.  i suggested that if he could obtain single cells we could
most likely measure the relative mitochondrial content in treated vs.
untreated rats.

does anybody have any experience in this area.  are there reagents
available, what fix and perm protocols have you used and are there any
references which may help.

thank you in advance for your help. responses via the cytometry mail list
or direct reponses are welcome and will be most appreciated.


Maurice (Mo) R.G. O'Gorman
Northwestern University
Chicago IL. aka dr.mrgo. for Dr. Robert Lane





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