fix and perm

Dr Vishal Kumar Gulati (Medicine) v.gulati at ic.ac.uk
Tue Mar 19 11:40:12 EST 1996


I am looking for a reagent 'fix and perm' used for fixing and permeablising
human lymphocytes for intracellular cytokine staining. 

I want to know other peoples experience with tihis reagent and also where
can I get it from in the UK and how much it costs.

I have been using Saponin but the results are not what i was expecting. what
are your views. Also what is better Brefeldin A or Monensin?

Thanks 

Vishal

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Dr. V. K. Gulati,
Deptt. Of Medicine,
Imperial College School of Medicine
London
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