PI dead cell gating/cell-cycle analysis

"facs1::isdellf"@rmslab.rockefeller.edu "facs1::isdellf" at rmslab.rockefeller.edu
Tue Mar 19 14:01:03 EST 1996


Hello fellow flowers,

I seem to recall a protocol for using propidium iodide for live/dead cell 
gating and DNA staining for cell-cycle analysis of the same sample.

would anyone be able to send me a refernce for this or an outline of a
protocol?

If this procedure is not possible, could somebody recommend one which they use?

I will be using a FACScan for data accquisition.

Thank you very much for your help



Frank Isdell
The Rockefeller University
1230 York Ave
NY
NY 10021
Tel: 212-327-7657
Fax: 212-327-8875




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