Quantifying LIVE CELLS

Libutti, Daniel A. dal7 at CIDDBD2.EM.CDC.GOV
Mon Mar 18 07:36:00 EST 1996


Deb;

Is that at 10^6/mL cell concentration?

Daniel
CDC
Atlanta, GA
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From: Deborah Berglund <umbbd at gemini.oscs.montana.edu>
Subject: Re: Quantifying LIVE CELLS
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A good viable cell label is Fluorescein diacetate.  It is taken up by 
live cells, broken down inside the cells by nonspecific esterases, at 
which point it becomes fluorescent.  I use a stock solution of 1mg/ml and 
use 10ul per ml of cells.

Deb Berglund
Montana State University






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