Live cell quantification.

James Weaver 301-594-5879 FAX 301-594-3037 WEAVER at
Fri Mar 15 14:25:42 EST 1996

I would second the idea of using an esterase substrate to measure live cells. I 
suggest that calcein-AM is  a better choice. It is very bright and is retained 
in live cells for many hours. Other labels such as CFDA can be lost in much less 
time. Since the emmision is also FITC-like, the possibility for 2-color 
live/dead labeling is still intact. Good luck.

-Jim Weaver

James L. Weaver				Email:	WEAVER at CDER.FDA.GOV
Molecular Pharmacology			Phone:	301-594-5879
Division of Research & Testing		FAX:	301-594-3037
OTR, CDER, Food & Drug Admin.		
Laurel, MD

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