Quantifying LIVE CELLS
dave at nucleus.immunol.washington.edu
Fri Mar 15 12:23:55 EST 1996
I am currently writing a chapter for Current Protocols in Cytometry on the
flow cytometric determination of cell viablity. I would very much appreciate
any comments on the fluorescent probe noted below as well as any comments on
other probes that you have found useful and--importantly--not useful.
Protocols as well as example illustrations are welcome. Any comments should
be sent within the next two weeks.
David M. Coder, Ph.D.
Editor, ISAC WWW Home Page
e-mail: dcoder at u.washington.edu
University of Washington
Seattle WA 98195-7650
Health Sciences Center H474A
University of Washington School of Medicine
1959 Pacific Street N.E.
Seattle WA 98195
Begin forwarded message:
Date: 13 Mar 1996 18:19:06 -0800
From: "Osama Nabulsi" <Osama.Nabulsi at amgen.com>
Subject: Quantifying LIVE CELLS
X-Mailer: Mail*Link SMTP/QM 3.0.0
REGARDING Quantifying LIVE CELLS
I am planning to run a survival bioassay were then I can quantitate LIVE
cells only using the flowcytometer.
I called Molecular Probes and they suggested "SYTOX" which stains dead cells
only (but will not cross the membrane of live cells). Have anyone used this
stain before? Advantages or Disadvantages?
or is there a live cell staining? Suggestions......
More information about the Cytometry