Quantifying LIVE CELLS

Antony Bakke bakkea at ohsu.EDU
Fri Mar 15 11:43:25 EST 1996

       One option for measuring dead cells is using an enzyme substrate
       like carboxy fluorescein diacetate succinimidyl ester.  This is
       cleaved by esters in the live cell to a form that is retained in the
       cell for many hours.  Dead cells lose their fluorescence quickly or
       never gain any fluorescence if already dead when the subtrate is

       Tony Bakke

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