Quantifying LIVE CELLS

Antony Bakke bakkea at ohsu.EDU
Fri Mar 15 11:43:25 EST 1996


       One option for measuring dead cells is using an enzyme substrate
       like carboxy fluorescein diacetate succinimidyl ester.  This is
       cleaved by esters in the live cell to a form that is retained in the
       cell for many hours.  Dead cells lose their fluorescence quickly or
       never gain any fluorescence if already dead when the subtrate is
       added.

       Tony Bakke




More information about the Cytometry mailing list