PI vs. 7-AAD Nuclear Staining

Dan Smith DJS at ALLP.COM
Fri Mar 15 13:24:00 EST 1996


I have been trying to surface stain apopotic MCF-7 breast cancer cells with 
Annexin V-FITC and stain the DNA with PI to test for necrosis vs apoptosis. 
 Even though the emission spectra shouldn't overlap I am having to 
compensate for what appears to be overlap in both FL1 and FL3.
Also the PI appears to be passing through the membrane of viable cells.
Any suggestions on either problem (Compensation or viability stain)
Would switching to 7-AAD be a possibility.
Thanks for the input,
Dan Smith
San Diego
djs at allp.com



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