Quantifying LIVE CELLS
AAllen1216 at aol.com
Fri Mar 15 11:45:11 EST 1996
>REGARDING Quantifying LIVE CELLS
>I am planning to run a survival bioassay were then I can quantitate LIVE
cells only >using the flowcytometer.
>I called Molecular Probes and they suggested "SYTOX" which stains dead cells
>only (but will not cross the membrane of live cells). Have anyone used
this stain >before? Advantages or Disadvantages?
>or is there a live cell staining? Suggestions......
Have you tried using a combination of FDA & PI? FDA will stain live cells
only and PI will stain dead cells. If you do a two color staining, you can
get an accurate count of live cells.
AAllen1216 at 1216.com
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