Quantifying LIVE CELLS

AAllen1216@aol.com AAllen1216 at aol.com
Fri Mar 15 11:45:11 EST 1996


 >REGARDING           Quantifying LIVE CELLS

>I am planning to run a survival bioassay were then I can quantitate LIVE
cells only >using the flowcytometer.  
>I called Molecular Probes and they suggested "SYTOX" which stains dead cells
 >only (but will not cross the membrane of live cells).  Have anyone used
this stain >before?  Advantages or Disadvantages?
>or is there a live cell staining? Suggestions......

Have you tried using a combination of FDA & PI?  FDA will stain live cells
only and PI will stain dead cells.  If you do a two color staining, you can
get an accurate count of live cells.

AAllen1216 at 1216.com





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