Quantifying LIVE CELLS

Libutti, Daniel A. dal7 at CIDDBD2.EM.CDC.GOV
Fri Mar 15 08:36:00 EST 1996


Fluorescein diacetate is a dye which is only taken up by live cells.  It is 
then cleaved within the cell to free the fluorescein resulting in the 
staining of only live cells.

Atlanta, GA
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Date: 13 Mar 1996 18:19:06 -0800
From: "Osama Nabulsi" <Osama.Nabulsi at amgen.com>
Subject: Quantifying LIVE CELLS
To: Cytometry Mailing List <cytometry at flowcyt.cyto.purdue.edu>
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  REGARDING           Quantifying LIVE CELLS

I am planning to run a survival bioassay were then I can quantitate LIVE =
cells only using the flowcytometer.  
I called Molecular Probes and they suggested "SYTOX" which stains dead =
cells  only (but will not cross the membrane of live cells).  Have anyone =
used this stain before?  Advantages or Disadvantages?
or is there a live cell staining? Suggestions......

Osama Nabulsi

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