Quantifying LIVE CELLS

Libutti, Daniel A. dal7 at CIDDBD2.EM.CDC.GOV
Fri Mar 15 08:36:00 EST 1996


Osama;

Fluorescein diacetate is a dye which is only taken up by live cells.  It is 
then cleaved within the cell to free the fluorescein resulting in the 
staining of only live cells.

Daniel
CDC
Atlanta, GA
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Date: 13 Mar 1996 18:19:06 -0800
From: "Osama Nabulsi" <Osama.Nabulsi at amgen.com>
Subject: Quantifying LIVE CELLS
To: Cytometry Mailing List <cytometry at flowcyt.cyto.purdue.edu>
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  REGARDING           Quantifying LIVE CELLS

I am planning to run a survival bioassay were then I can quantitate LIVE =
cells only using the flowcytometer.  
I called Molecular Probes and they suggested "SYTOX" which stains dead =
cells  only (but will not cross the membrane of live cells).  Have anyone =
used this stain before?  Advantages or Disadvantages?
or is there a live cell staining? Suggestions......

Osama Nabulsi
AMGEN INC.
(805)447-1594





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