Compensating cultured cells

Patricia L. Echeagaray echeagarayp at mail.ncifcrf.gov
Tue Mar 12 18:21:05 EST 1996


Occasionally, I need to run FACS on cultured cells (bone marrow,
specifically). I am challenged ad nauseum, at achieving a readable analysis
as I cannot seem to compensate-out the slant I seem to always get. I would
like to hear of any hints anyone might have in dealing with cells that
present in this manner. I use a B-D Facscan.

Thanks,

P. Echeagaray
P. Echeagaray




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