liposomes anyone?

Steve G. Hilliard hilliard at
Tue Mar 12 06:00:05 EST 1996

Hi folks,

I was approached the other day by a researcher who wants to analyze 
submicron membrane vesicles by flow.  They can detect the activity of 
membrane-bound ion channels using cuvets--they look at side-scatter, 
and see an increase in that signal when they add K+.  Over time the 
normal vesicles return to their normal size and SSC drops back to 
baseline.  (The plots look just like Ca++ or any other kinetics 
experiment!)  The increased side-scatter corresponds to shrinking of 
these vesicles.  Has anyone ever had any luck with these little guys?
I'm not even sure we'll be able to detect them, although I'm wondering 
about sticking them to larger beads, sort of like a carrier.
The thing that worries me is that one of the ultimate goals is to 
sort responders from non-responders, but you know what SSC looks 
like when the bimorph is on...  Anyone?

Steve (not really holding my breath on this one)
Steve G. Hilliard                Cell Analysis Facility           
University of Georgia

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