AFC assay.

R.T. SNOWDEN rts1 at
Fri Mar 8 11:46:08 EST 1996


       Does anyone have any experience in assaying intracellular  
benzyloxycarbonyl tetra peptide AFC (7-Amino-4-Trifluoromethyl 
Coumarin substrates.A few simple questions follow.

    1    Are these compounds generally cell permeable
    2     Can the cleaved AFC be excited at 352 nm , if so what is 
the emission wavelength.

    3      Does the cleaved AFC diffuse out of the cell.

             Thanks for any help

                                      Roger Snowden
 rts1 at

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