PKH and intracellular staining

Derek Davies davies at
Wed Mar 6 11:41:38 EST 1996

Hello all,

I was wondering if there is anyone out there who can help me.

We have used the membrane binding dye PKH26 to follow the division of 
keratinocytes with some success. Recently we have wanted to combine this 
with some intracellular staining for differentiation markers.

An initial experiment using saponin to permeabilise the cells was a tad 
disappointing in it appeared to abolish the membrane staining (the 
unpermeabilsed control was very bright). Has anyone had any ecxperience 
of this? Are there any vital refernces that I am missing?

Thanks in advance


Derek Davies
Imperial Cancer Research Fund
London, UK

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