cell cycle arrest

Roger Burger roger at cpd2.usu.edu
Tue Mar 5 19:56:00 EST 1996


Simon

Try Mitomycin C to arrest your cells.  It should do the trick.  Need a protocol?  email me direct.



>Hi
>
>All you cell cycle experts out there, can any one suggest ways of blocking
>cell cycle without killing cells. We would like our cells (T-cell lines) to
>remain arrested for several days without dying. We need the cells to be in
>G1, S or G2. We have tried gamma radiation and aphidicoline but the cells
>do not survive to the end of the assay.
>
>Simon Monard
>Aaron Diamond Center
>New York

***********************************************

Roger A. Burger         E-mail: Roger at cpd2.usu.edu
Research Immunologist
Center for Persons with Disabilities
Utah State University
Logan, UT  84322-6895
Voice: 801-797-2042
FAX:  801-797-2044





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