TdT assay

LI-JUN.MI@ccmail.med.nyu.edu LI-JUN.MI at ccmail.med.nyu.edu
Fri Mar 1 09:03:00 EST 1996


     Question #1
     
     Does TdT assay detect both single strand breaks and double strand 
     breaks? or it only detects double strand breaks?
     
     
     Question #2
     
     If cells are damaged with a lesion known to produce 3'-OH end in two 
     strands at different sites, how to differentiate the positive stained 
     cells are with the lesion, or with double strand breaks, or are 
     apoptotic cells, besides their position at SubG0 or at other phases 
     (e.g.G2)?  Or the lesion would be repaired and the half life is too 
     short to be detected?



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